GRP78 BiP Antibody
Source: Rabbit mAb Catalog No. : CY5167
GRP78 BiP Antibody (CY5167)
UniProt:P11021
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5167
数据表
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UniProt
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Mol Weight78kDa
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Alternative NamesGRP-78; GRP78; BIP; MIF2; HSPA5
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ImmunogenA synthesized peptide derived from human GRP78 BiP
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionWhen Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:200 dilution. -
Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution. -
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution. -
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution. -
Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:200 dilution. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Western blot analysis of GRP78 BiP expression in (1) LnCaP cell lysate;(2) HepG2 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody. -
Immunofluorescent analysis of HepG2 cells, using GRP78 BiP Antibody .
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