MEK1/2 Antibody
Source: Rabbit mAb Catalog No. : CY5168
MEK1/2 Antibody (CY5168)
UniProt:Q02750/P36507
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（4）
http://www.abways.cn/showproduct.asp?cid=CY5168
数据表
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UniProt
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Mol Weight44kDa
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Alternative NamesERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
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ImmunogenA synthesized peptide derived from human MEK1/2
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionDual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:5000~1:10000 IHC 1:100~1:500 ICC/IF 1:50~1:100 IP 1:50~1:100
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature. -
Western blot analysis of MEK1/2 expression in (1) 293T cell lysate; (2) A549 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody. -
Immunofluorescent analysis of Hela cells, using MEK1/2 Antibody . -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution. -
Immunofluorescent analysis using the Antibody at 1:500 dilution. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
引用文献（4条）
CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells-Animals
Chaetoglobosin G inhibits proliferation, autophagy and cell cycle of lung cancer cells through EGFR/MEK/ERK signaling pathway-pharmazie
FBXO18/Circrna211/Mir-431/CSF1 Axis Regulates the Establishment of Endometrial Receptivity Though Activating MAPK and CSF1R/PI3K/AKT/Mtor Pathways in Dairy Goats-research square
MiR-184 Combined with STC2 Promotes Endometrial Epithelial Cell Apoptosis in Dairy Goats via RAS/RAF/MEK/ERK Pathway-Genes (GENES-BASEL)
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