MCM2 Antibody
Source: Rabbit mAb Catalog No. : CY5200
MCM2 Antibody (CY5200)
UniProt:P49736
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5200
数据表
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UniProt
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Mol Weight125kDa
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Alternative NamesBM28; CCNL1; cdc19; CDCL1; cell devision cycle-like 1; cyclin-like 1; D3S3194; MCM2; MCM2 minichromosome maintenance deficient 2, mitotin;
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ImmunogenA synthesized peptide derived from human MCM2
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionThe minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork. This complex is also a key component of the pre-replication complex (pre-RC). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
Western blot analysis of MCM2 expression in HeLa cell lysate. -
Immunohistochemical analysis of paraffin-embedded mouse colon, using MCM2 Antibody. -
Immunofluorescent analysis using the Antibody at 1:50 dilution.
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