CD146 Antibody
Source: Rabbit mAb Catalog No. : CY5209
CD146 Antibody (CY5209)
UniProt:P43121
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5209
数据表
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UniProt
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Mol Weight72kDa
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Alternative NamesA32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein P1H12; Gicerin; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32;
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ImmunogenA synthesized peptide derived from human CD146
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionCD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Western blot analysis of CD146 expression in (1) A375 cell lysate;(2) HUVEC cell lysate. -
Immunohistochemical analysis of paraffin-embedded human uterus, using CD146 Antibody. -
Immunofluorescent analysis of A375 cells, using CD146 Antibody. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:200 dilution.
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