Phospho-p53 (S392) Antibody
Source: Rabbit mAb Catalog No. : CY5211
Phospho-p53 (S392) Antibody (CY5211)
UniProt:P04637
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5211
数据表
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UniProt
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Mol Weight53kDa
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Alternative NamesAntigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
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ImmunogenA synthesized peptide derived from human Phospho-p53 (S392)
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionTumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Western blot analysis of Phospho-p53 (Ser392) expression in HEK293 whole cell lysate. -
Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody. -
Immunofluorescent analysis using the Antibody at 1:150 dilution.
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