Smad5 Antibody
Source: Rabbit mAb Catalog No. : CY5213
Smad5 Antibody (CY5213)
UniProt:Q99717
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5213
数据表
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UniProt
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Mol Weight52kDa
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Alternative NamesDwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5;
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ImmunogenA synthesized peptide derived from human Smad5
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. Smad5 is a receptor-regulated Smad (R-Smad). Smad5 is required for normal development of the cardiovascular system in vivo; lack of the Smad5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of Smad5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:50 dilution. -
Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:50 dilution. -
Immunohistochemical analysis of paraffin-embedded Human stomach cancer, using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Western blot analysis of Smad5 expression in HEK293 cell lysate. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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