CD86 Antibody
Source: Rabbit mAb Catalog No. : CY5238
CD86 Antibody (CY5238)
UniProt:P42081
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5238
数据表
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UniProt
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Mol Weight70kDa
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Alternative NamesCD86;B7-2;B70;CD28LG2;LAB72;MGC34413; CTLA4 counter receptor B72; CLS1; Ly58; MB7;
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ImmunogenA synthesized peptide derived from human CD86
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of CD86 expression in Raji cell lysate.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody .
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Immunofluorescent analysis of K562 cells, using CD86 Antibody .



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