APAF1 Antibody _单克隆抗体-上海泊湾生物技术有限公司

APAF1 Antibody

Source: Rabbit mAb Catalog No. : CY5263

APAF1 Antibody  (CY5263)
UniProt:O14727
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5263

 Datasheet

数据表

UniProt

O14727
Mol Weight

135kDa
Alternative Names

APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413
Immunogen

A synthesized peptide derived from human APAF1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.

Applications

Reactivity

Human,Mouse
Application

WB,IHC,ICC/IF
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of APAF1 expression in (1) HeLa cell lysate; (2) MCF-7 cell lysate.
Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody .
Immunofluorescent analysis using the Antibody at 1:25 dilution.
Immunofluorescent analysis using the Antibody at 1:25 dilution.
Immunofluorescent analysis using the Antibody at 1:100 dilution.

引用文献（1条）

Circadian gene Clock participates in mitochondrial apoptosis pathways by regulating mitochondrial membrane potential, mitochondria out membrane permeablization and apoptosis factors in AML12 hepatocytes-Molecular and Cellular Biochemistry (MOL CELL BIOCHE