Cleaved PARP Antibody_单克隆抗体-上海泊湾生物技术有限公司

Cleaved PARP Antibody

Source: Rabbit mAb Catalog No. : CY5265

Cleaved PARP Antibody (CY5265)
UniProt:P09874
Application:WB,ICC/IF,IP,FC
Reactivity:Human
Source:Rabbit mAb
References（2）
http://www.abways.cn/showproduct.asp?cid=CY5265

 Datasheet

数据表

UniProt

P09874
Mol Weight

85kDa
Alternative Names

ADPRT; ADP-Ribosyltransferase; CPP32; PARP; PARP1; SCA1; PPOL; YAMA;
Immunogen

A synthesized peptide derived from human Cleaved PARP
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

Applications

Reactivity

Human
Application

WB,ICC/IF,IP,FC
Recommended dilution

WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Western blot analysis of Cleaved PARP expression in Jurkat cell lysate.
Immunofluorescent analysis of HeLa cells, using Cleaved PARP Antibody .
U2‐related proteins CHERP and SR140 contribute to colorectal tumorigenesis via alternative splicing regulation. -International Journal of Cancer
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution)

引用文献（2条）

U2‐related proteins CHERP and SR140 contribute to colorectal tumorigenesis via alternative splicing regulation.-International Journal of Cancer

Self-Assembled/Drug-Composed Nanomedicine for Synergistic Photonic Hyperthermia and Targeted Therapy of Breast Cancer by Inhibiting ERK, AKT, and STAT3 Signaling Cascades-VETERINARY RESEARCH(VET RES)