Histone H2A.X Antibody_单克隆抗体-上海泊湾生物技术有限公司

Histone H2A.X Antibody

Source: Rabbit mAb Catalog No. : CY5269

Histone H2A.X Antibody (CY5269)
UniProt:P16104
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5269

 Datasheet

数据表

UniProt

P16104
Mol Weight

14kDa
Alternative Names

H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; AW228881; H2A histone family member X; Histone 2A;
Immunogen

A synthesized peptide derived from human Histone H2A.X
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse spleen, using the Antibody at 1:500 dilution.
Western blot analysis of Histone H2A.X expression in Raji cell lysates.
Immunohistochemical analysis of paraffin-embedded rat lung, using Histone H2A.X Antibody.
Immunofluorescent analysis of Hela cells, using Histone H2A.X Antibody .
U2‐related proteins CHERP and SR140 contribute to colorectal tumorigenesis via alternative splicing regulation. -International Journal of Cancer

引用文献（1条）

U2‐related proteins CHERP and SR140 contribute to colorectal tumorigenesis via alternative splicing regulation.-International Journal of Cancer