B Raf Antibody
Source: Rabbit mAb Catalog No. : CY5319
B Raf Antibody (CY5319)
UniProt:P15056
Application:WB,IHC,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5319
数据表
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UniProt
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Mol Weight85kDa
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Alternative NamesB-RAF; p94; NS7; B-Raf proto-oncogene serine/threonine-protein kinase;BRAF;BRAF1;
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ImmunogenA synthesized peptide derived from human B Raf
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
Western blot analysis of B Raf expression in (1)Human brain lysate (2)HepG2 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody.
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