ROCK1 Antibody_单克隆抗体-上海泊湾生物技术有限公司

ROCK1 Antibody

Source: Rabbit mAb Catalog No. : CY5328

ROCK1 Antibody (CY5328)
UniProt:Q13464
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5328

 Datasheet

数据表

UniProt

Q13464
Mol Weight

158kDa
Alternative Names

p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated protein kinase 1;
Immunogen

A synthesized peptide derived from human ROCK1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

ROCK1 Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP,FC
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:150 dilution.
Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:150 dilution.
Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:150 dilution.
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:150 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Western blot analysis of ROCK1 expression in Ramos cell lysate.
Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody.
Immunofluorescent analysis of Hela cells, using ROCK1 Antibody .