Cytokeratin 20 Antibody
Source: Rabbit mAb Catalog No. : CY5332
Cytokeratin 20 Antibody (CY5332)
UniProt:P35900
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5332
数据表
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UniProt
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Mol Weight44kDa
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Alternative NamesCD20; CK-20; CK20; cytokeratin 20; Cytokeratin-20; K1C20; K20; keratin 20; Keratin, type I cytoskeletal 20; Keratin-20; KRT20; KRT21; MGC35423; Protein IT;
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ImmunogenA synthesized peptide derived from human Cytokeratin 20
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionIntermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract. Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium.
Applications
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ReactivityHuman,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:50
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Western blot analysis of Cytokeratin 20 expression in SW480 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human colon, using Cytokeratin 20 Antibody. -
Immunofluorescent analysis of Hela cells, using Cytokeratin 20 Antibody . -
Immunofluorescent analysis using the Antibody at 1:50 dilution.
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