Lactate Dehydrogenase Antibody
Source: Rabbit mAb Catalog No. : CY5348
Lactate Dehydrogenase Antibody (CY5348)
UniProt:P00338
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5348
数据表
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UniProt
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Mol Weight37kDa
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Alternative NamesLDHA; LDH-M; LDH1; PIG19; GSD11; TRG5; Proliferation inducing gene 19;
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ImmunogenA synthesized peptide derived from human Lactate Dehydrogenase
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionLactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Western blot analysis of LDHA expression in (1)Hela cell lysate;(2)Human heart lysate. -
Immunohistochemical analysis of paraffin-embedded human colon, using Lactate Dehydrogenase Antibody. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
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