Bak Antibody
Source: Rabbit mAb Catalog No. : CY5372
Bak Antibody (CY5372)
UniProt:Q16611
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5372
数据表
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UniProt
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Mol Weight23kDa
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Alternative NamesBAK1;BAK;BAK-LIKE;BCL2L7;CDN1;
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ImmunogenA synthesized peptide derived from human Bak
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionBak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.
Applications
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ReactivityHuman
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
Western blot analysis of extracts of HeLa cell lysate, using BAK1 antibody. -
Immunohistochemical analysis of paraffin-embedded mouse liver, using Bak Antibody. -
Immunofluorescent analysis of Hela cells, using Bak Antibody . -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution) -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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