Firefly Luciferase Antibody
Source: Rabbit mAb Catalog No. : CY5402
Firefly Luciferase Antibody (CY5402)
UniProt:P08659
Application:WB,ICC/IF
Reactivity:Firefly
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5402
数据表
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UniProt
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Mol Weight55kDa
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Alternative NamesLuciferase; Firefly; Luciferin 4 monooxygenase;
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ImmunogenA synthesized peptide derived from Firefly Luciferase
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionProduces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Applications
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ReactivityFirefly
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:1000~1:5000 ICC/IF 1:50~1:200
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Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate. -
Immunofluorescent analysis of 293 cells transfected with Firefly Luciferase, using Firefly Luciferase Antibody .
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