Hsc70 Antibody
Source: Rabbit mAb Catalog No. : CY5417
Hsc70 Antibody (CY5417)
UniProt:P11142
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5417
数据表
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UniProt
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Mol Weight70kDa
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Alternative NamesHeat shock cognate 71 kDa protein; HS7C; HSP73; HSP7C; HSPA10; HSPA8;
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ImmunogenA synthesized peptide derived from human Hsc70
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20 FC 1:30
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat ovary, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of Calreticulin expression in (1) HeLa cell lysate; (2) C6 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsc70 Antibody.
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Immunofluorescent analysis of MCF7 cells, using Hsc70 Antibody .
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.