Phospho-eIF4E (S209) antibody_单克隆抗体-上海泊湾生物技术有限公司

Phospho-eIF4E (S209) antibody

Source: Rabbit mAb Catalog No. : CY5419

Phospho-eIF4E (S209)  antibody (CY5419)
UniProt:P06730 
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5419

 Datasheet

数据表

UniProt

P06730
Mol Weight

25kDa
Alternative Names

CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E;
Immunogen

A synthesized peptide derived from human Phospho-eIF4E (S209)
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB 1:1000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Western blot analysis of eIF4E (phosphoS209) expression in (1) HEK293 cell lysate; (2) Mouse spleen lysate.
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Phospho-eIF4E (S209) antibody.