Alkaline Phosphatase Antibody
Source: Rabbit mAb Catalog No. : CY5421
Alkaline Phosphatase Antibody (CY5421)
UniProt:P05186
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5421
数据表
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UniProt
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Mol Weight57kDa
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Alternative NamesAlkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL;
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ImmunogenA synthesized peptide derived from human Alkaline Phosphatase
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionThere are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:100 IP 1:50 FC 1:50
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
Western blot analysis of Alkaline Phosphatase expression in (1)HepG2 cell lysate; (2)JAR cell lysate. -
Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody. -
Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody . -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution.
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