Brd4 Antibody
Source: Rabbit mAb Catalog No. : CY5423
Brd4 Antibody (CY5423)
UniProt:O60885
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5423
数据表
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UniProt
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Mol Weight152kDa
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Alternative NamesBrd4; CAP; HUNK1; MCAP; Bromodomain containing 4; chromosome associated protein;
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ImmunogenA synthesized peptide derived from human Brd4
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionBromodomain-containing protein 4 (BRD4) is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD3, and BRDT. BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues. BRD4 is a chromatin-binding protein with a preference for Lys14 on histone H3 as well as Lys5 and Lys12 on histone H4. BRD4 chromatin binding occurs throughout the cell cycle, including condensed mitotic chromosomes, when the majority of genes are silenced.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Western blot analysis of Brd4 expression in HeLa cell lysate. -
Immunohistochemical analysis of paraffin-embedded human kidney, using Brd4 Antibody. -
Immunofluorescent analysis of Hela cells, using Brd4 Antibody .
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