p53 Antibody
Source: Rabbit mAb Catalog No. : CY5465
p53 Antibody (CY5465)
UniProt:P04637
Application:WB,ICC/IF
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5465
数据表
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UniProt
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Mol Weight53kDa
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Alternative NamesAntigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53; TRP53;
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ImmunogenA synthesized peptide derived from human p53
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionPlays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.
Applications
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ReactivityHuman
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:500~1:2000 ICC/IF 1:50~1:200
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:50 dilution. -
Western blot analysis of p53 expression in (1) Raji cell lysate; (2) HepG2 cell lysate. -
Immunofluorescent analysis of Hela cells, using p53 Antibody .
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