Phospho-Cdk1/2 (T14) Antibody_单克隆抗体-上海泊湾生物技术有限公司

Phospho-Cdk1/2 (T14) Antibody

Source: Rabbit mAb Catalog No. : CY5489

Phospho-Cdk1/2 (T14) Antibody (CY5489)
UniProt:P06493/P24941
Application:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5489

 Datasheet

数据表

UniProt

P06493/P24941
Mol Weight

34kDa
Alternative Names

CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase;
Immunogen

A synthesized peptide derived from human Phospho-Cdk1/2 (T14)
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,IP
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea.
Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.