Rho A + B + C Antibody _单克隆抗体-上海泊湾生物技术有限公司

Rho A + B + C Antibody

Source: Rabbit mAb Catalog No. : CY5497

Rho A + B + C Antibody   (CY5497)
UniProt:P61586/P62745/P08134 
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5497

 Datasheet

数据表

UniProt

P61586/P62745/P08134
Mol Weight

22kDa
Alternative Names

ARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC;
Immunogen

A synthesized peptide derived from human Rho A + B + C
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

RhoA , RhoB , RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,FC
Recommended dilution

WB 1:500~1:2000 IHC 1:500~1:1000 ICC/IF 1:100~1:500 FC 1:200

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of Rho A + B + C expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody .
Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .