active + pro Caspase 3 Antibody
Source: Rabbit mAb Catalog No. : CY5501
active + pro Caspase 3 Antibody (CY5501)
UniProt:P42574
Application:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（5）
http://www.abways.cn/showproduct.asp?cid=CY5501
数据表
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UniProt
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Mol Weight17,32kDa
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Alternative NamesApopain precursor; CASP-3; CPP-32; CPP32; Caspase-3; Cysteine protease CPP32; ICE3; SCA-1; SREBP cleavage activity 1;
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ImmunogenA synthesized peptide derived from human active + pro Caspase 3
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,IP
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution. -
Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:100 dilution. -
Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:100 dilution. -
Western blot analysis of Calreticulin expression in (1) Jurkat cell lysate; (2) Jurkat whole cell lysates treated with staurosporine (1uM, 4 hours). -
Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody . -
Western blot analysis of Calreticulin expression in (1) Jurkat cell lysate; (2) Jurkat cells treated with 1uM staurosporine lysate. -
Data on the association of CMPK1 with clinicopathological features and biological effect in human epithelial ovarian cancer. -Cellular Signalling -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
引用文献（5条）
Data on the association of CMPK1 with clinicopathological features and biological effect in human epithelial ovarian cancer.-Cellular Signalling
Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin.-Clinical Epigenetics
Phenols fragment of Veronica ciliata Fisch. ameliorate free radical-induced nonalcoholic fatty liver disease by mediating PI3K/Akt signaling pathway-Journal of Ethnopharmacology (J ETHNOPHARMACOL)
Effects of chitinase-3-like protein 1 on brain death-induced hepatocyte apoptosis via PAR2-JNK-caspase-3-Biochemical and Biophysical Research Communications (BIOCHEM BIOPH RES CO)
The inhibitory effect Polygonum Cillinerve polysaccharide on transmissible gastroenteritis virus of swine-Research in Veterinary Science (RES VET SCI)
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