Cyclin B1 Antibody_单克隆抗体-上海泊湾生物技术有限公司

Cyclin B1 Antibody

Source: Rabbit mAb Catalog No. : CY5531

Cyclin B1 Antibody (CY5531)
UniProt:P14635
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse
Source:Rabbit mAb
References（2）
http://www.abways.cn/showproduct.asp?cid=CY5531

 Datasheet

数据表

UniProt

P14635
Mol Weight

55kDa
Alternative Names

CCN-2; CCNB; CCNB1; CCNB1-RS1; CYCB; CYCB1; G2/mitotic-specific cyclin B1;
Immunogen

A synthesized peptide derived from human Cyclin B1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF).

Applications

Reactivity

Human,Mouse
Application

WB,IHC,ICC/IF,FC
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:100

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:6k dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Western blot analysis of Cyclin B1 expression in HeLa cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody.
Immunofluorescent analysis of Hela cells, using Cyclin B1 Antibody .
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

引用文献（2条）

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Chaetoglobosin G inhibits proliferation, autophagy and cell cycle of lung cancer cells through EGFR/MEK/ERK signaling pathway-pharmazie