DGCR8 Antibody_单克隆抗体-上海泊湾生物技术有限公司

DGCR8 Antibody

Source: Rabbit mAb Catalog No. : CY5534

DGCR8 Antibody (CY5534)
UniProt:Q8WYQ5
Application:WB,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5534

 Datasheet

数据表

UniProt

Q8WYQ5
Mol Weight

100kDa
Alternative Names

DGCR8; DGCRK6; Gy1 ; C22orf12; D16Wis2; pasha;
Immunogen

A synthesized peptide derived from human DGCR8
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,ICC/IF,IP
Recommended dilution

WB 1:500~1:2000 ICC/IF 1:100~1:500 IP 1:50

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of DGCR8 expression in (1) HEK293 cell lysate; (2) HeLa cell lysate.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.