Histone H3.3 Antibody
Source: Rabbit mAb Catalog No. : CY5550
Histone H3.3 Antibody (CY5550)
UniProt:P84243
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5550
数据表
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UniProt
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Mol Weight15kDa
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Alternative NamesH3.3; H3.3A; H33_HUMAN ; H3F3; Histone H3.3 ; H3 histone family 3A; H3 histone family 3B
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ImmunogenA synthesized peptide derived from human Histone H3.3
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionVariant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:500~1:1000 IHC 1:500~1:1000 ICC/IF 1:500~1:1000
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Western blot analysis of Histone H3.3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate. -
Immunohistochemical analysis of paraffin-embedded mouse stomach, using Histone H3.3 Antibody .
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