AKT1 Antibody_单克隆抗体-上海泊湾生物技术有限公司

AKT1 Antibody

Source: Rabbit mAb Catalog No. : CY5551

AKT1 Antibody (CY5551)
UniProt:P31749
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（2）
http://www.abways.cn/showproduct.asp?cid=CY5551

 Datasheet

数据表

UniProt

P31749
Mol Weight

56kDa
Alternative Names

AKT; AKT1 kinase; C-AKT; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha; RAC-alpha serine/threonine kinase, kinase Akt1;
Immunogen

A synthesized peptide derived from human AKT1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP,FC
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Western blot analysis of AKT1 expression in MCF-7 cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.
Immunofluorescent analysis of Hela cells, using AKT1 Antibody.
Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation. -Oncology Reports
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.

引用文献（2条）

Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation.-Oncology Reports

Coptidis rhizoma and evodiae fructus against lipid droplet deposition in nonalcoholic fatty liver disease-related liver cancer by AKT-Chemical Biology & Drug Design