ATP citrate lyase Antibody
Source: Rabbit mAb Catalog No. : CY5560
ATP citrate lyase Antibody (CY5560)
UniProt:P53396
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5560
数据表
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UniProt
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Mol Weight122kDa
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Alternative NamesACL; ATPCL; CLATP; ATP citrate lyase;
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ImmunogenA synthesized peptide derived from human ATP citrate lyase
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:1000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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Western blot analysis of ATP citrate lyase expression in HeLa cell lysate. -
Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody . -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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