Akt(pan) 1/2/3 Antibody
Source: Rabbit mAb Catalog No. : CY5561
Akt(pan) 1/2/3 Antibody (CY5561)
UniProt:P31749/P31751/Q9Y243
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（10）
http://www.abways.cn/showproduct.asp?cid=CY5561
数据表
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UniProt
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Mol Weight56kDa
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Alternative NamesPKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3; Akt1/2/3; pan Akt;
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ImmunogenA synthesized peptide derived from human AKT1/2/3
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionAkt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:250 dilution. -
Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:250 dilution. -
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:1000 dilution. -
Western blot analysis of Akt(pan)1/2/3 expression in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate. -
Immunohistochemical analysis of paraffin-embedded human testis, using Akt(pan)1/2/3 Antibody. -
Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody. -
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry -
Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in RAW264.7 cells. -JOURNAL OF CELLULAR PHYSIOLOGY -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
引用文献（10条）
Argirein alleviates vascular endothelial insulin resistance through suppressing the activation of Nox4-dependent O2- production in diabetic rats.-Free Radical Biology and Medicine
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy.-Cellular Physiology and Biochemistry
Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in RAW264.7 cells.-JOURNAL OF CELLULAR PHYSIOLOGY
Salidroside induces apoptosis and protective autophagy in human gastric cancer AGS cells through the PI3K/Akt/mTOR pathway-Biomedicine & Pharmacotherapy (BIOMED PHARMACOTHER)
REC8 enhances stemness and promotes metastasis of colorectal cancer through BTK/Akt/β-catenin signaling pathway-Translational Oncology (TRANSL ONCOL）
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