EEA1 Antibody _单克隆抗体-上海泊湾生物技术有限公司

EEA1 Antibody

Source: Rabbit mAb Catalog No. : CY5563

EEA1 Antibody  (CY5563)
UniProt:Q15075
Application:WB,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5563

 Datasheet

数据表

UniProt

Q15075
Mol Weight

170kDa
Alternative Names

Early endosome antigen 1; EEA1; MST105; ZFYVE2;
Immunogen

A synthesized peptide derived from human EEA1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).

Applications

Reactivity

Human,Mouse,Rat
Application

WB,ICC/IF
Recommended dilution

WB 1:500~1:2000 ICC/IF 1:50~1:200

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of EEA1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.