DNA-PKcs Antibody
Source: Rabbit mAb Catalog No. : CY5618
DNA-PKcs Antibody (CY5618)
UniProt:P78527
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5618
数据表
-
UniProt
-
Mol Weight469kDa
-
Alternative NamesDNA-PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; EC 2.7.11.1; P460; PRKD; PRKDC; XRCC7, kinase DNA PK; DNA PKcs;
-
ImmunogenA synthesized peptide derived from human DNA-PKcs
-
StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
PurificationAffinity-chromatography
-
IsotypeRabbit IgG
-
ClonalityMonoclonal
-
DescriptionDNA-dependent protein kinase (DNA-PK) is an important factor in the repair of double-stranded breaks in DNA. Cells lacking DNA-PK or in which DNA-PK is inhibited fail to show proper nonhomologous end-joining (NHEJ). DNA-PK is composed of two DNA-binding subunits (Ku70 and Ku86) and one 450 kDa catalytic subunit (DNA-PKcs). It is thought that a heterodimer of Ku70 and Ku86 binds to double-stranded DNA broken ends before DNA-PKcs binds and is activated.
Applications
-
ReactivityHuman,Mouse,Rat
-
ApplicationWB,IHC,ICC/IF
-
Recommended dilutionWB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
-
Western blot analysis of DNA-PKcs expression in HeLa cell lysate. -
Immunohistochemical analysis of paraffin-embedded human breast, using DNA-PKcs Antibody. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
销售咨询
一键电话