Phospho-ER alpha (S118) Antibody_单克隆抗体-上海泊湾生物技术有限公司

Phospho-ER alpha (S118) Antibody

Source: Rabbit mAb Catalog No. : CY5624

Phospho-ER alpha (S118) Antibody (CY5624)
UniProt:P03372
Application:WB,IHC,ICC/IF
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5624

 Datasheet

数据表

UniProt

P03372
Mol Weight

66kDa
Alternative Names

ESR1; Era; Eralpha; Estrogen receptor; Estradiol receptor; ER-alpha; Estrogen receptor 1; NR3A1; ER; ESR; ESRA; Estrogen receptor alpha;
Immunogen

A synthesized peptide derived from human ER alpha
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ERα activity. ERα is phosphorylated on multiple sites.

Applications

Reactivity

Human
Application

WB,IHC,ICC/IF
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of Phospho-ER alpha (S118) expression in (1) MCF7 cell lysate; (2) MCF7 cell lysate treated with b-Estradiol and EGF.
Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-ER alpha (S118) Antibody.
Immunofluorescent analysis of MCF7 cells treated with EGF, using Phospho-ER alpha (S118) Antibody.
Immunofluorescent analysis using the Antibody at 1:25 dilution.
Immunofluorescent analysis using the Antibody at 1:25 dilution.
Immunofluorescent analysis using the Antibody at 1:100 dilution.