ATG7 Antibody
Source: Rabbit mAb Catalog No. : CY5658
ATG7 Antibody (CY5658)
UniProt:O95352
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5658
数据表
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UniProt
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Mol Weight78kDa
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Alternative NamesATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7;
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ImmunogenA synthesized peptide derived from human ATG7
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionThe molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
Applications
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ReactivityHuman
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:10000~1:50000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20 FC 1:20
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. -
Western blot analysis of ATG7 expression in HepG2 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human colon, using ATG7 Antibody. -
Immunofluorescent analysis using the Antibody at 1:150 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution.
引用文献（1条）
Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells-Cancers
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