Phospho-Hsp27 (S82) Antibody
Source: Rabbit mAb Catalog No. : CY5701
Phospho-Hsp27 (S82) Antibody (CY5701)
UniProt:P04792
Application:WB,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5701
数据表
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UniProt
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Mol Weight27kDa
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Alternative Names28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27;
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ImmunogenA synthesized peptide derived from human Phospho-Hsp27 (S82)
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionHeat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IP
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Recommended dilutionWB 1:500~1:1000 IP 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock.
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