TOP1 Antibody
Source: Rabbit mAb Catalog No. : CY5725
TOP1 Antibody (CY5725)
UniProt:P11387
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5725
数据表
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UniProt
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Mol Weight91kDa
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Alternative NamesDNA topoisomerase 1; DNA topoisomerase I; TOP1; TOPI; topoisomerase I; type I DNA
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ImmunogenA synthesized peptide derived from human TOP1
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionReleases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
Applications
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ReactivityHuman,Mouse
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature. -
Western blot analysis of TOP1 expression in Jurkat cell lysate. -
Immunohistochemical analysis of paraffin-embedded human colon, using TOP1 Antibody.
引用文献（1条）
MicroRNA-24-3p promotes proliferation and inhibits apoptosis of porcine granulosa cells by targeting P27-Journal of Integrative Agriculture
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