Caspase-3 p12 Antibody
Source: Rabbit mAb Catalog No. : CY5783
Caspase-3 p12 Antibody (CY5783)
UniProt:P42574
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5783
数据表
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UniProt
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Mol Weight12,29,32kDa
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Alternative NamesApopain; CASP-3; CASP3; Caspase-3 subunit p12; CPP-32; Cysteine protease CPP32; Protein Yama; SCA-1; SREBP cleavage activity 1;
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ImmunogenA synthesized peptide derived from human Caspase-3 p12
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionCaspase-3 is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Involved in the activation cascade of caspases responsible for apoptosis execution.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:500~1:1000 IHC 1:100~1:500 ICC/IF 1:50~1:200
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Caspase-3 p12 Antibody.
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Immunofluorescent analysis of HeLa cells, using Caspase-3 p12 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.