Cyclin E1 Antibody_单克隆抗体-上海泊湾生物技术有限公司

Cyclin E1 Antibody

Source: Rabbit mAb Catalog No. : CY5815

Cyclin E1 Antibody (CY5815)
UniProt:P24864
Application:WB,ICC/IF
Reactivity:Human
Source:Rabbit mAb
References（3）
http://www.abways.cn/showproduct.asp?cid=CY5815

 Datasheet

数据表

UniProt

P24864
Mol Weight

47kDa
Alternative Names

CCNE; Ccne1; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; G1/S specific cyclin E; G1/S-specific cyclin-E1;
Immunogen

A synthesized peptide derived from human Cyclin E1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Cyclin E1 and cyclin E2 can associate with and activate CDK2. Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3.

Applications

Reactivity

Human
Application

WB,ICC/IF
Recommended dilution

WB 1:1000~1:2000 ICC/IF 1:50~1:200

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Western blot analysis of Cyclin E1 expression in HeLa cell lysate.
Immunofluorescent analysis of Hela cells, using Cyclin E1 Antibody.
APC/C is essential for hematopoiesis and impaired in aplastic anemia. -Oncotarget

引用文献（3条）

APC/C is essential for hematopoiesis and impaired in aplastic anemia.-Oncotarget

KIF15 Promotes Progression of Castration Resistant Prostate Cancer by Activating EGFR Signaling Pathway-Frontiers in Oncology (Front. Oncol)

Deficiency in WDFY4 reduces the number of CD8+ T cells via reactive oxygen species-induced apoptosis-Molecular Immunology (MOL IMMUNOL)