Phospho-Cyclin E1 (T77) Antibody
Source: Rabbit mAb Catalog No. : CY5816
Phospho-Cyclin E1 (T77) Antibody (CY5816)
UniProt:P24864
Application:WB,IHC,ICC/IF
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5816
数据表
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UniProt
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Mol Weight48kDa
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Alternative NamesCCNE; Ccne1; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; G1/S specific cyclin E; G1/S-specific cyclin-E1;
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ImmunogenA synthesized peptide derived from human Cyclin E1
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionCyclin E1 and cyclin E2 can associate with and activate CDK2. Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3.
Applications
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ReactivityHuman
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
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Western blot analysis of Phospho-Cyclin E1 (T77) expression in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.
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