ATF4 Antibody_单克隆抗体-上海泊湾生物技术有限公司

ATF4 Antibody

Source: Rabbit mAb Catalog No. : CY5873

ATF4 Antibody (CY5873)
UniProt:P18848
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5873

 Datasheet

数据表

UniProt

P18848
Mol Weight

50kDa
Alternative Names

Cyclic AMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67;
Immunogen

A synthesized peptide derived from human ATF4
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

ATF4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway. ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4. Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP,FC
Recommended dilution

WB 1:500~1:1000 IHC 1:50~1:200 ICC 1:100~1:500 IP 1:50 FC 1:50

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of ATF4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
Immunohistochemical analysis of paraffin-embedded mouse kidney, using ATF4 Antibody.
Immunofluorescent analysis of 293 cells, using ATF4 Antibody.
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution)