Phospho-AKT1 (T450) Antibody
Source: Rabbit mAb Catalog No. : CY5885
Phospho-AKT1 (T450) Antibody (CY5885)
UniProt:P31749
Application:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5885
数据表
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UniProt
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Mol Weight60kDa
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Alternative NamesAKT 1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha;
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ImmunogenA synthesized peptide derived from human AKT1
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionAkt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,IP
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Recommended dilutionWB 1:1000~1:2000 IHC 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Western blot analysis of Phospho-AKT1 (T450) expression in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate.
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