Hsp27 Antibody_单克隆抗体-上海泊湾生物技术有限公司

Hsp27 Antibody

Source: Rabbit mAb Catalog No. : CY5934

Hsp27 Antibody (CY5934)
UniProt:P04792
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5934

 Datasheet

数据表

UniProt

P04792
Mol Weight

27kDa
Alternative Names

28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27;
Immunogen

A synthesized peptide derived from human Hsp27
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP,FC
Recommended dilution

WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Western blot analysis of Hsp27 expression in HeLa cell lysate.
Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1. -ONCOLOGY LETTERS
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

引用文献（1条）

Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1.-ONCOLOGY LETTERS