Phospho-Raf1 (S259) Antibody
Source: Rabbit mAb Catalog No. : CY5944
Phospho-Raf1 (S259) Antibody (CY5944)
UniProt:P04049
Application:WB,IHC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY5944
数据表
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UniProt
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Mol Weight73kDa
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Alternative NamesC-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;
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ImmunogenA synthesized peptide derived from human Phospho-Raf1 (S259)
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionOnce activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC
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Recommended dilutionWB 1:500~1:1000 IHC 1:50~1:100
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. -
Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A. -
Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin. -Clinical Epigenetics
引用文献（1条）
Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin.-Clinical Epigenetics
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