Ubiquitin Antibody
Source: Rabbit mAb Catalog No. : CY5965
Ubiquitin Antibody (CY5965)
UniProt:P0CG47
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5965
数据表
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UniProt
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Mol WeightkDa
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Alternative Namesubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A;
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ImmunogenA synthesized peptide derived from human Ubiquitin
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionPlays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
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Western blot analysis of Ubiquitin expression in HepG2 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Ubiquitin Antibody. -
Immunofluorescent analysis of Jurkat cells, using Ubiquitin Antibody. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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