Phospho-NF- kappaB p65 (Ser276) Antibody
Source: Rabbit pAb Catalog No. : CY6367
Phospho-NF- kappaB p65 (Ser276) Antibody (CY6367)
UniProt:Q04206
Application:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit pAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY6367
数据表
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UniProt
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Mol Weight65kDa
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Alternative NamesNFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65
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ImmunogenA synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Serine 276
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityPolyclonal
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DescriptionNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,IP
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Recommended dilutionWB 1:500-1:2000 IHC 1:50-1:200 IP
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Western blot analysis of extracts from HeLa cells. Line1: Using NF-κB p65 (Phospho-Ser276) Antibody; Line2: The same antibody preincubated with synthesized non-phospho- peptide; Line3: The same antibody preincubated with synthesized phosphopeptide. -
Western blot analysis of NF- kappaB p65 phosphorylation expression in Jurkat cell lysate,The lane on the right is treated with the antigen-specific peptide. -
Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using NF-κB p65(Phospho-Ser276) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
引用文献（1条）
Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation-MOLECULAR MEDICINE
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