Phospho-NF- kappaB p65 (Ser311) Antibody
Source: Rabbit pAb Catalog No. : CY6369
Phospho-NF- kappaB p65 (Ser311) Antibody (CY6369)
UniProt:Q04206
Application:WB,IHC
Reactivity:Human,Mouse
Source:Rabbit pAbhttp://www.abways.cn/showproduct.asp?cid=CY6369
数据表
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UniProt
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Mol Weight75kDa
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Alternative NamesNFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65
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ImmunogenA synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Serine 311
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityPolyclonal
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DescriptionNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
Applications
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ReactivityHuman,Mouse
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ApplicationWB,IHC
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Recommended dilutionWB 1:500-1:2000 IHC 1:50-1:200
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Western blot analysis of extracts from 293 cells treated with TNF-α (20ng/ml, 30mins), using NF-κB p65 (phospho-Ser311) antibody (Line 1 and 2). -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (phospho-Ser311) antibody.
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