Phospho-NPM (Thr199) Antibody
Source: Rabbit pAb Catalog No. : CY6384
Phospho-NPM (Thr199) Antibody (CY6384)
UniProt:P06748
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit pAbhttp://www.abways.cn/showproduct.asp?cid=CY6384
数据表
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UniProt
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Mol Weight32 / 38kDa
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Alternative NamesB23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, nucleophosmin
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ImmunogenA synthesized peptide derived from human NPM around the phosphorylation site of Threonine 199
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityPolyclonal
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Descriptiona nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
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Western blot analysis of extracts from HepG2 cells, using NPM (Phospho-Thr199) antibody. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NPM (Phospho-Thr199) antibody. -
Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.
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