Dnmt1 Antibody_单克隆抗体-上海泊湾生物技术有限公司

Dnmt1 Antibody

Source: Rabbit mAb Catalog No. : CY6649

Dnmt1 Antibody (CY6649)
UniProt:P26358
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（1）
http://www.abways.cn/showproduct.asp?cid=CY6649

 Datasheet

数据表

UniProt

P26358
Mol Weight

183kDa
Alternative Names

ADCADN; CXXC finger protein 9; CXXC9; DNA methyltransferase 1; DNA MTase; Dnmt1o; HSN1E; M.HsaI; MCMT; Met1; MommeD2;
Immunogen

A synthesized peptide derived from human Dnmt1
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF
Recommended dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat spleen, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:100 dilution.
Western blot analysis of Dnmt1 expression in (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

引用文献（1条）

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function-Cell Research (CELL RES)