Phospho-POLR2A (S2) Antibody
Source: Rabbit mAb Catalog No. : CY6847
Phospho-POLR2A (S2) Antibody (CY6847)
UniProt:P24928
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY6847
数据表
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UniProt
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Mol Weight270kDa
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Alternative NamesPOLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS;
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ImmunogenA synthesized peptide derived from human POLR2A
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionDuring transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
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Western blot analysis of Phospho-POLR2A (S2) expression in (1) MCF7 cell lysate treated with Lambda Phosphatase; (2) MCF7 cell lysate. -
Immunohistochemical analysis of paraffin-embedded human spleen, using Phospho-POLR2A (S2) Antibody. -
Immunofluorescent analysis of MCF7 cells, using Phospho-POLR2A (S2) Antibody . -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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