Atg7(Apg7) Antibody
Source: Rabbit mAb Catalog No. : CY7051
Atg7(Apg7) Antibody (CY7051)
UniProt:O95352
Application:WB,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY7051
数据表
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UniProt
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Mol Weight77kDa
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Alternative NameshAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L;
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ImmunogenA synthesized peptide derived from human Atg7(Apg7)
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionFormation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,ICC/IF
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Recommended dilutionWB 1:1000~1:5000 ICC/IF 1:50~1:200
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of Atg7(Apg7) expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate; (3) Rat kidney lysate.